Application

This Anti-Glutamate (No Glutaraldehyde) Antibody is validated for use in IH for the detection of Glutamate (No Glutaraldehyde).

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & ReceptorsNeuroinflammation & Pain

Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.1% - 2.5% glutaraldehyde for optimum detection.

*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5018 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

Optimal working dilutions must be determined by the end user.

DILUTION: Prepare enough of the AB5018 for your days use by diluting 100X with 1% GSPBT.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Glutamate-glutaraldehyde

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

IgG fraction in sterile 0.1M phosphate buffer. No preservative.

Format: Purified

Specificity

Glutamate (L-Glutamate). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (﹤1:1000) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (﹤1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) against D-glutamate. Significant cross-reactivity (1:8) against free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue).

Storage and Stability

Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze.